TABLE 1.
Antibodies used in this study
| Primary antibody | Source | Dilution (v/v) | Secondary antibody | Source | Dilution (v/v) | Technique |
|---|---|---|---|---|---|---|
| Chicken anti-myelin P0 | Novus Biologicals (NB100-1607) | 1:100 | IgG Rabbit anti-chicken biotin conjugated | Acris Antibodies (R1300B) | 1:300 | IC-Pa |
| Rabbit anti–3-NT | Millipore (AB5411) | 1:200 | IgG goat anti-rabbit biotin conjugated | Chemicon-Millipore (AQ132B) | 1:300 | IC-Pa |
| Rabbit anti-Kir4.1 | Chemicon (AB5818) | 1:200 | IgG donkey anti-rabbit Alexa Fluor 488 | Molecular Probes (A21206) | 1:400 | IHF |
| Mouse anti–β-actin | Sigma-Aldrich (A5441) | 1:5000 | IgG sheep anti-mouse HRP conjugated | GE Healthcare (NA931) | 1:5000 | WB |
| Mouse anti–α-tubulin | Sigma-Aldrich (T9026) | 1:2500 | IgG sheep anti-mouse HRP conjugated | GE Healthcare (NA931) | 1:20,000 | WB |
| Mouse anti-CBS | Abnova (H00000875-A01) | 1:2500 | IgG sheep anti-mouse HRP conjugated | GE Healthcare (NA931) | 1:20,000 | WB |
| Rabbit anti-BHMT | González et al. [37] | 1:5000 | IgG goat anti-rabbit HRP conjugated | Bio-Rad Laboratories (170-6515) | 1:5000 | WB |
| Rabbit anti-ADK | Abcam (ab88903) | 1:500 | IgG goat anti-rabbit HRP conjugated | Bio-Rad Laboratories (170-6515) | 1:5000 | WB |
| Rabbit anti-Hcy | Chemicon-Millipore (AB5512) | 1:1000 | IgG goat anti-rabbit HRP conjugated | Bio-Rad Laboratories (170-6515) | 1:3000 | WB |
| Rabbit anti-p22phox | Santa Cruz Biotechnology (sc-20781) | 1:500 | IgG goat anti-rabbit HRP conjugated | Bio-Rad Laboratories (170-6515) | 1:3000 | WB |
| Rabbit anti-MnSOD | Upstate Millipore (06-984) | 1:2500 | IgG goat anti-rabbit HRP conjugated | Bio-Rad Laboratories (170-6515) | 1:3000 | WB |
| Rabbit anti-NOX3 | Abcam (ab82708) | 1:1000 | IgG goat anti-rabbit HRP conjugated | Bio-Rad Laboratories (170-6515) | 1:5000 | WB |
| Goat anti-AHCY | Santa Cruz Biotechnology (sc-66759) | 1:1000 | IgG donkey anti-goat HRP conjugated | Santa Cruz Biotechnology (sc-2020) | 1:10,000 | WB |
| Goat anti-MTR | Abnova (PAB6022) | 1:250 | IgG donkey anti-goat HRP conjugated | Santa Cruz Biotechnology (sc-2020) | 1:10,000 | WB |
| Goat anti-ADA | Santa Cruz Biotechnology (sc-7451) | 1:2500 | IgG donkey anti-goat HRP conjugated | Santa Cruz Biotechnology (sc-2020) | 1:10,000 | WB |
| Goat anti-NOX4 | Santa Cruz Biotechnology (sc-21860) | 1:500 | IgG donkey anti-goat HRP conjugated | Santa Cruz Biotechnology (sc-2020) | 1:3000 | WB |
The table lists the antibodies used in this work indicating the dilutions used for the different techniques. IC-P, immunohistochemistry-paraffin; IHF, immunohistofluorescence; WB, Western blotting. aTertiary extrAvidin peroxidase-conjugated antibody (1:300 v/v; Sigma-Aldrich) was needed in this case.