Figure 6.

Nonglycosylated F3 associates more strongly with GRP94 and less strongly with CNX and CRT. A) ARPE-19 cells expressing F3 variants were cross-linked and lysed, and F3 was IP’d, followed by Western blot analysis. In order to robustly observe the F3 interaction with the lectins, an MOI of 25 was necessary. Inputs represent 2% of the IP’d material. Similar relative amounts of F3 were observed in the MOI 5 and 25 inputs. Representative data of 3 independent experiments. B) Quantification of GRP94 binding to F3 by LI-COR. Data are presented as the ratio of GRP94/F3 and are normalized to the GRP94/F3 ratio of WT F3, n = 3 (*P < 0.05, t test).