Figure 1. Vegetative and reproductive phenotypes of CENH3 complemented lines.

(A) Plants at rosette stage from different complemented lines compared to wild-type Columbia (WT) and GFP-tailswap, a high frequency haploid inducer [20]. The genotype of the endogenous CENH3 locus is indicated in parentheses. LoCENH3 is L. oleraceum CENH3 and BrCENH3 is B. rapa CENH3. AtNTT-LoHFD and LoNTT-AtHFD are chimeric CENH3s described in the key. (B) Anthers stained for viability with Alexander stain. Viable pollen granules stain purple. (C) Measures of fertility based on number of seeds per silique and seed appearance. Bars in different shades of grey represent counts from different T1 lines. For each measurement, seeds from 5 siliques were pooled and counted.