Fig. 5.
Quantification of Babesia duncani ITS1 DNA in infected hamsters by real-time PCR (RT-PCR) and ddPCR. (A) DNA copy numbers measured by RT-PCR (black bars) and ddPCR (gray bars) were transformed to log10 values. Non-transformed copy numbers are shown in Appendix: Supplementary Table S1. Data represent the means ± standard deviations of each sample tested in triplicate. Samples that were undetectable by both assays at 3 days p.i. in hamster 3 are not shown. (B) Parasitemia of B. duncani -infected hamsters determined by microscopy.