Table 1.
Primers and probes used in this study.
| Designation | Target | Sequence (5′–3′) |
|---|---|---|
| Primera | ||
| BABITS-F | 3′ region of the B. microti or B. duncani 18S rRNA gene | GGTGAACCTGCRGAAGGATC |
| BABITS-R | 5′ region of the B. microti or B. duncani 28S rRNA gene | TCTKCCGCTTARTTATATGC |
| BmITS1-F | B. microti ITS1 | TCCCATTTGGGTTACGCTGG |
| BmITS1-R2_mb | B. microti ITS1 | CGTGCAGACAAACCCGCCTT |
| BdITS1-F | B. duncani ITS1 | GCTTCCTAACCCGAGACCAA |
| BdITS1-R | B. duncani ITS1 | CACTGGCGGGGTGAAAAGTA |
| Probeb | ||
| BmITS1-P | B. microti ITS1 | AGTGGCCTTGGACGTAGCGGC |
| BdITS1-P | B. duncani ITS1 | TACGGCCCCTGATGTCGGGG |
a
Expected amplicon sizes are as follows: BABITS-F/BABITS-R: 930–950 bp; BmITS1-F/BmITS1-R2_mb: 142 bp; BdITS1-F/BdITS1-R: 125 bp.
b
Probes were dye-labeled with FAM™. PCR Primers were purchased from IDT. Probes were purchased from Biosearch Technologies.