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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Exp Parasitol. 2014 Dec 8;149:24–31. doi: 10.1016/j.exppara.2014.12.003

Table 2.

Real-time PCR detection and quantitation of Babesia DNA standards.

B. microti
standarda 		Cq B. duncani
standarda 		Cq
Mean SD Mean SD
1 × 108 12.85 0.155 1 × 108 10.90 0.194
1 × 107 16.36 0.059 1 × 107 14.15 0.257
1 × 106 19.82 0.075 1 × 106 17.39 0.058
1 × 105 23.44 0.042 1 × 105 21.06 0.202
1 × 104 27.03 0.049 1 × 104 24.78 0.112
1 × 103 30.54 0.184 1 × 103 28.53 0.414
100 34.24 0.529 100 31.71 0.127
10 37.60 0.408 10 34.13 1.057
1 0.0 0.0 1 0.0 0.0
H2O 0.0 0.0 H2O 0.0 0.0
a

Values reflect copy numbers per reaction based on serial dilutions of plasmid DNA standard by DNA concentration and known molecular mass. Data represent the means ± standard deviations of each dilution tested in triplicate from a representative experiment of three performed.