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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Exp Parasitol. 2014 Dec 8;149:24–31. doi: 10.1016/j.exppara.2014.12.003

Table 3.

ddPCR detection and quantitation of Babesia DNA standards.

B. microti
standarda 		ddPCR valueb B. duncani
standarda 		ddPCR valueb
Mean SD Mean SD
1 × 105 38,200 721 1 × 105 51,733 1,858
1 × 104 3,393 170 1 × 104 4,827 50
1 × 103 344 26.5 1 × 103 414 31.4
100 38.5 3.8 100 44.3 3.4
10 1.2 0 10 7.5 1.1
1 0 0 1 0 0
H2O 0 0 H2O 0 0
a

Values reflect copy numbers added per reaction based on serial dilutions of plasmid DNA standard by DNA concentration and known molecular mass.

b

Copies per reaction detected. Data represent the means ± standard deviations of each dilution tested as five replicates from a representative experiment of three performed.