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. Author manuscript; available in PMC: 2016 Feb 15.
Published in final edited form as: Dev Biol. 2014 Dec 16;398(2):255–266. doi: 10.1016/j.ydbio.2014.12.008

Fig. 6. NEKL-2 and NEKL-3 reporters are expressed in the hypodermis.

Fig. 6

(A-D) DIC and corresponding fluorescence images of NEKL-3::GFP expression in embryos. Expression is first detected in hypodermal cells coincident with the initiation of embryonic morphogenesis (A), with expression increasing in the hypodermis during the remainder of embryogenesis (B-D). Embryonic stages depicted are bean (A, ~350 min post fertilization), 1.5-fold (B, ~420 min), 3- fold (C, ~520 min) and pretzel (D, >600 min). In adults (E) and larvae (not shown), NEKL-3::GFP is present in the apical region of the parts of hyp7 that do not overlie body muscles and in the apical region of other hypodermal cells, but expression is not evident in the seam cells. The expression of NEKL-3::GFP can be globular, punctate or slightly tubular. (F-H) NEKL-2 (F) and NEKL-3 (G) reporters show little overlap in subcellular localization (H, white), although NEKL-2::mCherry is expressed in puncta near the apical surface of hyp7. Scale bar in A = 10 µm for A-D; in E = 10 µm; in F = 5 µm for F-H.