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. 2015 Jan 12;35(3):675–683. doi: 10.3892/ijmm.2015.2065

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Copyright © 2015, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Representative methylation-specific PCR (MSP) of S100P promoter status. (A) MSP analysis of methylated control DNA (mDNA), unmethylated control DNA (uDNA) and untreated DNA (wDNA) using designed primers sets showing the specificity of primers. (B) MSP analysis of S100P in pancreatic cancer and paired normal tissue. (C) Representative results of MSP analysis of S100P in polypoid and ulcerative colon cancer and paired normal mucosa samples. The PCR products in lanes M and U indicate the presence of methylated and unmethylated templates, respectively. Ma, 100-bp ladder marker.