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. 1976 Dec;73(12):4309–4313. doi: 10.1073/pnas.73.12.4309

Endonuclease recognition sites mapped on Zea mays chloroplast DNA

John R Bedbrook 1, Lawrence Bogorad 1
PMCID: PMC431441  PMID: 16592373

Abstract

The closed-circular DNA molecules of 85 × 106 daltons from Zea mays chloroplasts were isolated, digested with the restriction endonucleases Sal I, Bam I, and EcoRI, and the resulting fragments sized by agarose gel electrophoresis. A map of maize chloroplast DNA showing the relative location of all the Sal I recognition sequences and many of the Bam I and EcoRI recognition sites was determined. A DNA sequence representing approximately 15% of the Zea mays chloroplast genome is repeated. The two copies of this sequence are in an inverted orientation with respect to one another and are separated by a nonhomologous sequence representing approximately 10% of the genome length. The inverted repeats contain the genes for chloroplast ribosomal RNAs.

Keywords: chloroplast rRNAs, Sal I, Bam I, EcoRI, hybridization

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Selected References

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