Figure 1.

Flow cytometric analysis of cell surface connexin levels in primary human acute myeloid leukemia (AML) cells. The AML cell levels of Cx32, Cx37, Cx43 and Cx45 were determined for 38 unselected patients and the Cx26 levels for 16 unselected patients. The results are presented as the percentage of positive cells for each individual patient compared with the corresponding negative unstained control. The positive cell regions were defined for each individual patient based on the forward angle light scatter (FSC)/right angle side scatter (SSC) diagrams and contained <2% and >1% of the cells of the unstained negative control. Each dot represents a single patient, and the median level is indicated by a vertical line.