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. 2014 Dec 19;35(3):645–652. doi: 10.3892/ijmm.2014.2045

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Copyright © 2015, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Hierarchical clustering analysis based on the expression of Cx32, Cx37, Cx43 and Cx45 in acute myeloid leukemia (AML) cells. Connexin expression was analysed by flow cytometry in 38 patients, and the analysis is based on the percentage of positively stained cells for each connexin and patient. An unsupervised hierarchical clustering was performed using the complete linkage method and Euclidean distance. The results are presented as denograms with corresponding heat maps. The darker red color indicates higher expression. The data obtained in the clustering are compared with the FAB classification of the patients (see right part of the figure).