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. 2015 Jan 16;35(3):625–636. doi: 10.3892/ijmm.2015.2070

Figure 5.

Figure 5

Western blot analysis of NF-κB expression. (A-D) The mRNA expression levels of IκBα and IκBβ were detected by RT-qPCR at 3 time points for each group. (E) The mRNA expression levels of IκBα and IκBβ in pancreatic tissue were decreased by AS-IV. Western blot analysis of NF-κB p65 in the nucleus, and IκBα, IκBβ in the whole protein at 3 time points for each group. Lamin A and β-actin were regarded as the internal reference for nuclear proteins and whole proteins, respectively. The administration of AS-IV increased the mRNA and protein expression levels of IκBα and IκBβ, and inhibited the activation of NF-κB. Figures are representative of 3 independent experiments. Data are represented as the means ± SD from 3 independent experiments. *P<0.05 compared with the normal control group at the same time point; ▽P<0.05 compared with the NaTc/L-Arg + vehicle-treated group at the same time point. AS-IV, astragaloside IV; NaTc, sodium taurocholate; L-Arg, L-arginine; NF-κB, nuclear factor-κB.