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. 2015 Jan 16;35(3):625–636. doi: 10.3892/ijmm.2015.2070

Figure 8.

Figure 8

Effects of AS-IV on AP in vitro. Necrosis and proliferation of pancreatic acinar cells was detected by the depletion of ATP and CCK-8 assay, respectively. AS-IV suppressed the dose-dependent depletion of ATP in acinar cells treated with NaTc/L-Arg (A and C), and increased the dose-dependent proliferation of acinar cells by CCK-8 (B and D). (E) The expression of NF-κB p65 in the nucleus, and IκBα, IκBβ, SOD1 and SOD2 in the cytoplasm of pancreatic acinar cells was detected by western blot analysis. Data are represented as the means ± SD from 3 independent experiments. *P<0.05 compared with the normal control group at the same time point; ▽P<0.05 compared with the NaTc-treated group at the same time point; △P<0.05 compared with the NaTc/L-Arg + 40 μmol/l AS-IV-treated group. AS-IV, astragaloside IV; ATP, adenosine triphosphate; SOD1, manganese superoxide dismutase; SOD2, cuprum/zinc superoxide dismutase; NaTc, sodium taurocholate; L-Arg, L-arginine; AP, acute pancreatitis; NF-κB, nuclear factor-κB; CCK-8, cell counting kit-8.