Figure 6.

Adpa-Mn inhibits tumor growth in vivo. Hepatocellular carcinoma (Hep-A) 1×10⁷ tumor cells (grown in donor mice) were transplanted subcutaneously into the armpits of ICR mice. One day after transplantion, the mice were randomly allocated to either the control or treatment groups, with 10 mice in each group. Drugs were administered intraperitoneally on days 0-9. After the mice were sacrificed, solid tumors were separated. (A and B) Tumors were photographed and weighed. (C and D) Lean body weight and thymus splenic index were calculated. (E) Paraffin-embedded sections of tumor tissues from mice were analyzed by H&E staining. Scale bar, 50 μm. (F) Protein from tumor tissue from mice in each group was extracted and analyzed by western blot analysis. Data represent the means ± SEM. n=10, ^*p<0.05 and ^**p<0.01 vs. control.