Skip to main content
. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Genomics. 2014 Nov 28;105(2):108–115. doi: 10.1016/j.ygeno.2014.11.007

Fig. 3. AEBP2 binding to the promoter regions of Aebp2, Jarid2 and Snai2.

Fig. 3

The potential binding of AEBP2 to the three promoter regions was confirmed through performing individual ChIP experiments using the chromatin prepared from the thymus of one-month-old adult mice. Each locus was analyzed first by PCR at a fixed number of 40 cycles using a set of templates derived from Input, IgG, anti-Aebp2 antibody (left panel). This was further analyzed with qPCR showing the enrichment levels relative to that of Input with error bars (right panel). The enrichment levels showed 10 fold (Aebp2), 13 fold (Jarid2), and 4 fold (Snai2) higher than those of IgG. This further confirmed the binding of AEBP2 to the promoter regions of Aebp2, Jarid2 and Snai2.