Skip to main content
. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Genomics. 2014 Nov 28;105(2):108–115. doi: 10.1016/j.ygeno.2014.11.007

Fig. 5. AEBP2 involvement in cell migration.

Fig. 5

A) Scratch assay. HeLa cells were individually transfected with the following constructs: pcDNA empty, Aebp2 somatic form (Aebp2-S), and Aebp2 embryonic form (Aebp2-e). After a scratch was formed on a layer of confluent cells using p10 pipet tips, the migration of the cells was monitored. The HeLa cells expressing the somatic form showed a two-fold increase in cell migration compared to the cells with the pcDNA empty vector 18 hours after the scratch formation. However, the cells with the embryonic form did not show any difference compared to the cells with the pcDNA empty vector. B) Agarose droplet assay. The HEK 293T cells transfected individually with pcDNA-eGFP, Aebp2 somatic-eGFP and Aebp2 embryonic-eGFP were trapped in the 0.25% agarose droplets containing 0.5% FBS. After one-week incubation, the cells with Aebp2 somatic-eGFP migrated out of the agarose droplets, confirming the enhancing effect of the somatic form on cell migration. However, the cells with both pcDNA-eGFP and Aebp2 embryonic-eGFP did not migrate out of the droplets.