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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Cell Signal. 2014 Oct 29;27(1):147–155. doi: 10.1016/j.cellsig.2014.10.009

Figure 3.

Figure 3

Phosphorylation of ERKs in wild-type cells stimulated with cAMP but without vortexing. Cells were prepared, treated, and analyzed as described in Figure 2 except that cAMP stimulation was conducted while cells were continuously shaken at 200 rpm in conical tubes (no vortexing). (A) Wild-type cells stimulated with 10 μM cAMP. (B) Wild-type cells stimulated with 100 nM cAMP. (C) Wild-type cells not stimulated with cAMP. (D) Graph of the mean pixel intensity of the phosphorylated ERK1 bands of three immunoblots from multiple experiments. Gray and black shaded and open columns correspond to treatments (A), (B), and (C), respectively. Error bars represent the standard deviation.