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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Cell Signal. 2014 Oct 29;27(1):147–155. doi: 10.1016/j.cellsig.2014.10.009

Figure 6.

Figure 6

Phosphorylation of ERKs in erk1−, gα4− chimeric and clonal cell populations stimulated with folate. Cells were prepared and stimulated with folate as described in figure 5. (A) Chimeric population of erk1− and gα4− cells mixed in a 1:1 ratio and stimulated with 50 μM folate. (B) erk1− cells only stimulated with 50 μM folate. (C) gα4− cell only stimulated with 50 μM folate. (D) Graph of the mean pixel intensity of the phosphorylated ERK1 bands of three or more immunoblots of multiple experiments (*values have been normalized to the 0 min time mean intensity). Gray and black shaded columns correspond to treatments (A) and (C), respectively. Error bars represent the standard deviation.