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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Anal Biochem. 2014 Dec 20;472:62–66. doi: 10.1016/j.ab.2014.12.006

Figure 2.

Figure 2

A) Protein yield from a 48-year-old Caucasian male donor bone for 400 mM ammonium phosphate dibasic at room temperature (RT) and 75°C and 400 mM ammonium phosphate dibasic/200 mM ammonium bicarbonate at RT or 75°C. B) Protein yield for at 75°C for 400 mM ammonium phosphate dibasic/200 mM ammonium bicarbonate, 400 mM ammonium phosphate dibasic, 200 mM ammonium bicarbonate, and 400 mM ammonium phosphate dibasic/200 mM ammonium bicarbonate/4M guanidine HCl. C) Protein yield for all other human samples extracted using 400 mM ammonium phosphate dibasic/200 mM ammonium bicarbonate at 75°C. *p<0.05 **p<0.01 ***p<0.001