Fig. 4.

Data from Fig. 3 were projected into the Δ[Ca²⁺]_Free,I, [Ca²⁺]_Free plane. A. The solid curve is Eq. 7 with parameters from in Fig. 3A. B. The solid curve is Eq. 7 with parameters from Fig. 3B. As in Fig. 3, the addition of a second buffer improved the fit. Note that the data in the two panels are scaled differently according to the respective K_d,D values from Fig. 3A versus Fig. 3B.