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. Author manuscript; available in PMC: 2016 Feb 2.
Published in final edited form as: Mol Pharm. 2015 Jan 15;12(2):621–629. doi: 10.1021/mp5006883

Figure 9. Quantification of DNA uptake in MCF-7/Adr cell line.

Figure 9

(A) Flow cytometry quantified P(SiDAAr)5P3 polyplexes' DNA uptake levels in MCF-7/Adr cell line. 1/1/4 polyplexes showed over 2-fold higher DNA uptake than 1/O/2 polyplexes. The level dropped in 1/2/6 polyplexes due to polymer masking. (B) Confocal microscopy visualized YOYO-1-DNA's cellular internalization. 1/1/4 polyplexes showed a stronger DNA internalization than 1/O/2 polyplexes. Fluorescence intensity dropped in 1/2/6 polyplexes, confirming the flow cytometry results. Images were captured using 80× objective lens. Bar=50 μm.