Table 2. Average hydrodynamic diameter (Dh) (nm) and zeta potential (mV) of PEI and P(SiDAAr)5P3 polyplexes at different weight ratios.
| DNA/siRNA/PEI | Size, mean (SD) | PDI, mean (SD) | Zeta, mean (SD) |
|---|---|---|---|
| 1/O/1 | 276.33 (156.16) | 0.44 (0.21) | 40.58 (5.99) |
| 1/1/2002 | 301.33 (148.34) | 0.34 (0.25) | 39.05 (8.72) |
| 1/0.5/1.5 | 454.2 (159.56) | 0.29 (0.10) | 38.3 (8.95) |
| 1/2/2003 | 385.77 (142.15) | 0.34 (0.21) | 38.83 (3.20) |
| 1/O/2 | 313.4 (80.77) | 0.37 (0.17) | 48.19 (9.39) |
| 1/1/2004 | 438.57 (127.17) | 0.23 (0.18) | 36.93 (10.10) |
| 1/0.5/3 | 312.83 (6.34) | 0.28 (0.05) | 53.56 (23.09) |
| 1/2/2006 | 355.5 (84.76) | 0.29 (0.15) | 67.15 (18.04) |
| DNA/siRNA/ P(SiDAAr)5P3 | Size, mean (SD) | PDI, mean (SD) | Zeta, mean (SD) |
| 1/O/1 | 289.1 (22.89) | 0.24 (0.04) | 3.90 (2.50) |
| 1/1/2002 | 386.3 (47.34) | 0.37 (0.15) | -2.44 (18.09) |
| 1/0.5/1.5 | 307.17 (65.69) | 0.24 (0.14) | 14.30 (15.80) |
| 1/2/2003 | 302.07 (55.46) | 0.29 (0.35) | 20.17 (7.25) |
| 1/O/2 | 280.47 (17.12) | 0.28 (0.04) | 21.17 (14.51) |
| 1/1/2004 | 283.93 (29.41) | 0.26 (0.10) | 22.69 (19.38) |
| 1/0.5/3 | 306.5 (14.35) | 0.29 (0.06) | 22.59 (5.95) |
| 1/2/2006 | 318.5 (44.29) | 0.31 (0.07) | 17.87 (8.83) |
| siRNA/DNA/ P(SiDAAr)5P3 | Size, mean (SD) | PDI, mean (SD) | Zeta, mean (SD) |
| 1/O/2 | 233.43 (17.96) | 0.25 (0.07) | 20.44 (15.04) |
| 1/0.5/3 | 228.13 (10.86) | 0.19 (0.06) | 10.96 (1.03) |
| 1/1/2004 | 271.97 (86.71) | 0.31 (0.23) | 29.08 (15.92) |
| 1/2/2006 | 296.67 (85.45) | 0.30 (0.14) | 18.25 (7.54) |
PDI: Polydispersity Index, D/S/P: DNA/siRNA/polymer, S/D/P: siRNA/DNA/polymer.