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. 2014 Nov 18;4(1):25–38. doi: 10.1016/j.molmet.2014.11.001

Figure 4.

Figure 4

Reduced engraftment of chronic HFD fetal liver cells in the HFD male niche. (A) Schematic for non-competitive transplantation of HFD and control diet fetal liver cells. (B) Peripheral blood fetal liver-derived donor chimerism for non-competitive transplantation at 4 and 14 weeks post-transplant. (C) Schematic for competitive transplantation of HFD versus control fetal liver cells into diet-conditioned recipients. (D) Body weights of recipient mice after 11 weeks of nutritional preconditioning, just prior to transplantation. Peripheral blood chimerism of (E) HFD or (F) control diet fetal liver cells in diet-conditioned recipients at 4 weeks post-transplant. (G) Comparison of HFD fetal liver chimerism in graft pairs; duplicate grafts were given to 2 recipients of the same sex, which were conditioned on different diets. Blood lineage chimerism for residual recipient CD45.1, control donor fetal liver CD45.2, and HFD donor fetal liver CD45.1/.2 cells in (H) B220+, (I) CD3+, and (J) Mac-1+/Gr-1+ populations. HFD, high-fat diet; cGy, centigray; F, female; M, male. Error bars reflect standard error of the mean. Asterisks indicate P ≤0.05 for * and P ≤0.01 for **.