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. 2014 Dec 4;4(2):93–105. doi: 10.1016/j.molmet.2014.11.006

Figure 5.

Figure 5

Conditioned media (CM) from WT, but not sR3 SWAT macrophages inhibit adipogenic differentiation of 3T3-L1 cells. (A) Images of terminally differentiated 3T3-L1 adipocytes stained with BODIPY 493/503 (green, lipid droplets), Syto60 (red, cytosol) and Hoechst (blue, nuclei, scale bar = 100 μm). (B) Quantification showed a significant decrease in adipogenic differentiation of the adipocytes treated with CM from WT macrophages, compared to adipocytes treated with sR3 macrophage CM (n = 4 each, two-tailed Student's t-test, **P < 0.01, ***P < 0.001). (C) Decreased (IL-6) or absent (IL-17, GM-CSF, IL-1α) proinflammatory cytokines in CM of sR3 SWAT macrophages. IL-10 (a Th2 cytokine) was only detectable in sR3 macrophage CM (n = 4 each). All data are mean ± SD.