Fig 5.

A-E, Representative images of ALI cultures from healthy control subjects of involucrin-positive staining (brown, ×400 magnification). Results are shown for untreated conditions (Fig 5, A) and treatment with 100 nmol/L DK-PGD₂ for 48 hours (Fig 5, B), and 100 nmol/L DK-PGD₂ plus 1 μmol/L AZD6430 for 48 hours (Fig 5, C). D, TGF-β1 (10 ng/mL) for 72 hours. E, TGF-β1 (10 ng/mL) plus AZD6430 (1 μmol/L) for 72 hours. F, Representative image of involucrin-positive staining for an ALI culture from an untreated asthmatic patient. G, Dot blot to show quantitation of involucrin staining for cultures with 48- and 72-hour treatments. P values are based on 1-way ANOVA. Overall P < .001. P values shown in the figure are based on the Tukey post hoc test.