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. 2014 Nov 17;3:252. Originally published 2014 Oct 27. [Version 2] doi: 10.12688/f1000research.5512.2

Figure 10. Investigation of interactions amongst GraS, GraX, GraR and BSA.

Figure 10.

( A) GST-GraS was immobilized onto the glutathione resin and incubated with GraR. The resin was washed seven times, and finally eluted three times with 10 mM glutathione. Lanes: M, marker; GraS, protein prior to immobilization; GraR, protein prior to incubation with resin; In, incubation mixture in the absence of resin; U, unbound fraction; W1 and W7, fractions collected through washes of the resin; E1, E2, E3, three subsequent elution fractions collected when resin was incubated with elution buffer. ( B) GST-GraS was immobilized onto the glutathione resin and incubated with BSA. Similar protocol of washing and elution was used as in ( A). ( C) GST-GraS was immobilized onto the glutathione resin followed by the protocol given in ( A). ( D) GST was immobilized onto the glutathione resin, followed by the protocol given in ( A). ( E) GraR was immobilized onto the Ni-NTA resin, washing and elutions were done as in ( A).