Figure 13. DNase I footprint analysis of P vraFG with GraR and GraR C (non-coding strand).
P vraFG (10 ng) labeled on the non-coding strand was incubated with increasing concentrations of GraR or GraR C and subjected to DNase I. The DNA sequence protected by GraR is shown on the right. The dashed lines indicate the binding sites, and the solid lines show the palindromic sequence in DNA, as suggested by Dintner et al. 21.