Table 1. Sequence of the primers used in this study.
Name | Primer Sequence (5′-3′)* | Restrict.
enzyme |
---|---|---|
BceR Fwd | GC CATATGTTGTTTAAACTTTTGCTGATTGAAG | NdeI |
BceR Rev | GC GAATTCTTAATCATAGAACTTGTCCTCTTCCTTC | EcoRI |
GraR Fwd | GC CATATGATGCAAATACTACTAGTAGAAGATGACAATAC | NdeI |
GraR Rev | ACG AAGCTTTTATTATTCATGAGCCATATATCCTTTTCCTA | HindIII |
GraR CFwd | GC CATATGGCTGAAGAAAAACGTACATTGACTTGG | NdeI |
GraR CRev | CG AAGCTTTTATTCATGAGCCATATATCCTTTTCC | HindIII |
BceS Fwd | G GTCGACTCGCGTTTTATAAAAGCTTGAAAAC | SalI |
BceS Rev | GC GCGGCCGCTCACACGCTTATGACATGTTC | NotI |
GraS Fwd | ACG GGATCCGAAATAGAAGAAATTAAACATAAAGATTTAG | BamHI |
GraS Rev | ACG CTCGAGTTTCATTTTGTAATGGGAAAATCAATC | XhoI |
HGraS Fwd | CACCGAAATAGAAGAAATTAAACATAAAGATTTAG | |
HGraS Rev | TTAAAATGACAAATTTGTCACTTCCG | |
HGraX Fwd | AGCT AAGCTTATGAAACCTAAAGTTTTATTAGCAG | HindIII |
HGraX Rev | TCGA CTCGAGTCATTTAGTATATTTCATATTTTCTCC | XhoI |
GraS CAFwd | GGATCCATGTATTTTGATTACGTGTCACTT | BamHI |
GraS CARev | CTCGAGTTAAAATGACAAATTTGTCACTT | XhoI |
GraS DHp-CAFW | G GGATCCGTTGTTGAGCAACAGTTACAAT | BamH1 |
GraS DHp-CARev | CTCGAGTTAAAATGACAAATTTGTCACTT | XhoI |
GraX Fwd | ACG CATATGAAACCTAAAGTTTTATTAGCAGG | NdeI |
GraX Rev | ACG AAGCTTTTATCATTTAGTATATTTCATATTTTCTCC | HindIII |
VraF FW | CATG CCATGGTGGCAATTTTAGAAGTAAAAC | NcoI |
VraF Rev | CG GGATCCTTAAAGGTCATAATTAACGCC | BamHI |
VraFmFW | GGTGGCGTTAATTATGACCTTTTAGGATCCCCGAATTCGAGCTCC | |
VraFmRev | GGAGCTCGAATTCGGGGATCCTAAAAGGTCATAATTAACGCCACC |
*Italicized sequences indicate the restriction sites. Abbreviations: Fwd, forward primer; RE, restriction enzyme; Rev, reverse primer