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. 2014 Nov 17;3:252. Originally published 2014 Oct 27. [Version 2] doi: 10.12688/f1000research.5512.2

Table 1. Sequence of the primers used in this study.

Name Primer Sequence (5′-3′)* Restrict.
enzyme
BceR Fwd GC CATATGTTGTTTAAACTTTTGCTGATTGAAG NdeI
BceR Rev GC GAATTCTTAATCATAGAACTTGTCCTCTTCCTTC EcoRI
GraR Fwd GC CATATGATGCAAATACTACTAGTAGAAGATGACAATAC NdeI
GraR Rev ACG AAGCTTTTATTATTCATGAGCCATATATCCTTTTCCTA HindIII
GraR CFwd GC CATATGGCTGAAGAAAAACGTACATTGACTTGG NdeI
GraR CRev CG AAGCTTTTATTCATGAGCCATATATCCTTTTCC HindIII
BceS Fwd G GTCGACTCGCGTTTTATAAAAGCTTGAAAAC SalI
BceS Rev GC GCGGCCGCTCACACGCTTATGACATGTTC NotI
GraS Fwd ACG GGATCCGAAATAGAAGAAATTAAACATAAAGATTTAG BamHI
GraS Rev ACG CTCGAGTTTCATTTTGTAATGGGAAAATCAATC XhoI
HGraS Fwd CACCGAAATAGAAGAAATTAAACATAAAGATTTAG
HGraS Rev TTAAAATGACAAATTTGTCACTTCCG
HGraX Fwd AGCT AAGCTTATGAAACCTAAAGTTTTATTAGCAG HindIII
HGraX Rev TCGA CTCGAGTCATTTAGTATATTTCATATTTTCTCC XhoI
GraS CAFwd GGATCCATGTATTTTGATTACGTGTCACTT BamHI
GraS CARev CTCGAGTTAAAATGACAAATTTGTCACTT XhoI
GraS DHp-CAFW G GGATCCGTTGTTGAGCAACAGTTACAAT BamH1
GraS DHp-CARev CTCGAGTTAAAATGACAAATTTGTCACTT XhoI
GraX Fwd ACG CATATGAAACCTAAAGTTTTATTAGCAGG NdeI
GraX Rev ACG AAGCTTTTATCATTTAGTATATTTCATATTTTCTCC HindIII
VraF FW CATG CCATGGTGGCAATTTTAGAAGTAAAAC NcoI
VraF Rev CG GGATCCTTAAAGGTCATAATTAACGCC BamHI
VraFmFW GGTGGCGTTAATTATGACCTTTTAGGATCCCCGAATTCGAGCTCC
VraFmRev GGAGCTCGAATTCGGGGATCCTAAAAGGTCATAATTAACGCCACC

*Italicized sequences indicate the restriction sites. Abbreviations: Fwd, forward primer; RE, restriction enzyme; Rev, reverse primer