Effect of Ca2+ (A) and (B), and Mg2+ (C) and (D) concentration on MH release from Ca2+- or Mg2+-based complexes, fabricated by mixing 1.2 mg/ml DS, 1 mg/ml MH, and different concentrations of metal ions. Three different release media were used: HBSS without BSA, HBSS with 0.175 and 40 mg/ml of BSA. Data shown are average ± STD (n=3).