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. 2015 Jan 27;15:7. doi: 10.1186/s12861-015-0058-9

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© De Silva et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Purification of HR+ cells by FACS in early pregnancy. (A) The luminal population of mammary epithelial cells (CD24^hiCD49f^lo) was separated into hormone-sensing cells (Sca1^hi CD49b^lo; purple gate) and alveolar progenitor cells (Sca1^lo CD49b^hi; orange gate). (B) Quantitative RT-PCR on 500 directly-lysed cells per sample for markers that distinguish the two luminal cell types. Values are relative to mRNA levels in HR+ cells from virgin animals and normalised to HPRT expression. Error bars indicate standard deviation for 3 individual mice.