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. 2014 Dec 15;8(2):169–181. doi: 10.1242/dmm.017285

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© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 5. — HIF knockdown induces TAK-IKK activity. (A) HeLa cells were transfected with an siRNA control or an siRNA against HIF-1α and then treated with 10 ng/ml of TNF-α for the indicated times. Whole-cell lysates were prepared and analysed by western blotting for the indicated proteins (‘p’ prefix denotes phosphorylated protein). (B) HeLa, MDA-MB-231 and U2OS were transfected with an siRNA control or an siRNA against HIF-1α and treated with TNF-α for the indicated times. Whole-cell lysates were prepared and analysed by western blotting for the indicated proteins. (C) HeLa cells were transfected with the indicated siRNA oligonucleotides before treatment with TNF-α for 30 min before harvest. Whole-cell lysates were analysed by western blotting for the depicted proteins. (D) HeLa cells were transfected with the indicated siRNA oligonucleotides before harvest. Whole-cell lysates were analysed by western blotting for the indicated proteins. See also supplementary material Fig. S4.