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In vitro fluorescence images of fluorescein-NHS dye, Cy5.5-NHS dye and the probe Ab-FL-Cy5.5 under different imaging conditions: (A) Excitation wavelength,470 nm; emission wavelength, 530 nm and (B) excitation wavelength, 610 nm; emission wavelength, 700 nm. NHS, N-hydroxysuccinimide.
