Figure 2.

Expression of miR-99a in A549 and H1299 cells inhibits cell proliferation and colony formation by inducing G₁-G₀ cell cycle arrest. (A) A549 and H1299 mocks, stably transfected with miR-NC and miR-99a, were assessed using MTT assays to determine the proliferation of A549 or H1299 cells. The data are presented as the mean ± standard deviation from three independent experiments. (B) Colony formation assays were performed to determine the proliferation of A549 or H1299 cells. The colonies were counted and images of the colonies were captured. (C) Histograms for red fluorescence quantified by flow cytometry of A549 and H1299 mock cells, which stably expressed miR-NC or miR-99a. ^*P<0.05. miR-99a, microRNA-99a; miR-NC, miR negative control; OD, optical density.