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Western blot of corpora cavernosa smooth muscle cells. The western blot shows the effect of 4PB after 48 h exposure at concentrations of 0 (lane 1), 1 mM (lane 2), and 5 mM (lane 3). The reporter protein used was tubulin along with Cx43. Three examples are shown. From top to bottom – increased expression, no change and reduced expression. The average value for six Western blots using ImageJ analysis was 0.93 ± 0.25 SD of controls.
