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Nested methylation-specific polymerase chain reaction detection of methylated NDRG4 gene in carcinoma tissues, feces, urine and blood of a representative colorectal cancer case (patient no. 3). T, carcinoma tissues; S, stool; B, blood; U, urine; M, methylated products (88 bp); U, unmethylated products (100 bp); 3, patient no. 3; M-p, positive control of methylated DNA; U-p, positive control of unmethylated products; NC, negative control; bp, base pairs.
