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. 2014 Dec 18;9(3):1109–1115. doi: 10.3892/ol.2014.2813

Figure 1.

Figure 1

Principle of multispectral imaging technology in analyzing the expression level of miRNA. (A and B) Applying the CRi-Nuance™ Multispectral Imaging System. Dyes with a wavelength between 420 and 720 nm (NBT/BCIP and methyl green) were acquired as references (microphotographs at ×50 magnification) and (C) microphotographs of tissues that underwent ISH, including colorectal adenocarcinoma (CA) tissues, were spectrally resolved to detect the expression level of miR-92a (magnification, ×200). NBT/BCIP, nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate; ISH, in situ hybridization; miR/miRNA, microRNA.