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. 2014 Dec 18;9(3):1109–1115. doi: 10.3892/ol.2014.2813

Figure 4.

Figure 4

Multispectral imaging analysis of the expression level of miR-92a in 34 samples of colorectal adenocarcinoma (CA) and its precancerous lesions (CA, n=13; CA-N, n=12; HGIN, n=11; HGIN-N, n=10; LGIN, n=10; and LGIN-N, n=9). (A) The average expression level of miR-92a in the cytoplasm of the tissue, determined by pixel area. (B) The average expression level of miR-92a in the nuclei of the tissue, determined by pixel area. (C) The average expression intensity of miR-92a in the cytoplasm of the tissue, determined by OD. (D) The nucleus-to-cytoplasm ratio of miR-92a expression in each group. (E) The expression of miR-92a in the nuclei of each group. The above data is calculated by the formula mentioned in the Materials and methods section. Each sample was repeated three times and the error bar represents the standard deviation. *P<0.05; **P<0.01. CA, colorectal carcinoma; CA-N, CA paralesional normal tissue; HGIN, high-grade intraepithelial neoplasia; HGIN-N, HGIN paralesional normal tissue; LGIN, low-grade intraepithelial neoplasia; LGIN-N, LGIN paralesional normal tissue; NBT/ BCIP, nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate; OD, optical density.