Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Feb 2;208(3):299–311. doi: 10.1083/jcb.201410076

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Roelants et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 1. — Gin4 negatively regulates Fpk1-dependent phosphorylation of Ypk1 and Dnf1. (A) Wild-type (WT) strain (BY4741) or an isogenic gin4Δ mutant (YAT100) expressing Ypk1-myc from the GAL1 promoter (pAM76) were grown to mid-exponential phase, then either mock-treated or treated with Myr (1.25 µM) as indicated, collected, and lysed. The resulting extracts were resolved by SDS-PAGE and analyzed by immunoblotting with anti–c-myc mAb 9E10. (B) As in A, except that wild-type cells (Y258) carried both a plasmid expressing Ypk1-myc from the GAL1 promoter (pAM54) and either an empty vector (BG1805) or the same vector expressing Gin4 (pGin4-zz) from the GAL1 promoter. Gin4-zz was detected via its zz tag using an anti-HA mAb as a source of IgG. (C) As in A, except that a wild-type strain (BY4741) or isogenic gin4Δ mutant (YAT100), fpk1Δ fpk2Δ double mutant (YFR205), or fpk1Δ fpk2Δ gin4Δ triple mutant (YFR278) were used and only the Myr-treated cells are shown. (D) Same as in C, except that the cells expressed Dnf1(1403–1571)-myc from plasmid pES10 and the extract was analyzed using a Phos-tag gel.