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. 2015 Feb 2;208(3):299–311. doi: 10.1083/jcb.201410076

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© 2015 Roelants et al.

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Figure 5. — Gin4 and Fpk1 colocalize prominently at the site of bud emergence. (A) Cells expressing Gin4-mCherry (YFR355) were viewed by time-lapse fluorescence microscopy at ∼30-min intervals. (B) As in A, for cells coexpressing Gin4-mCherry and Myo1-GFP (YFR388) at the indicated times. (C) As in B, for cells coexpressing Myo1-mCherry (YFR385) and Fpk1-GFP from the TPI1 promoter on a CEN plasmid (pFR150). (D) As in B, for cells coexpressing Fpk1-GFP (pFR150) and Gin4-mCherry (YFR355). (E, top) Same cells as in B. (E, bottom) Same cells as in D. Broken lines represent the perimeter of the indicated cells. Bars, 2 µm.