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. 2015 Feb 2;208(3):273–281. doi: 10.1083/jcb.201408056

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© 2015 Bentley et al.

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Figure 5. — Identifying the domain of KIF13B that binds to endosomes. (A) As shown in the schematic, different regions of KIF13B tail tagged with FRB were coexpressed with tdTM-BicD2⁵⁹⁴-FKBP and TfR-GFP. (B) In control cells, TfR-GFP was distributed throughout the cell. After treatment with the linker drug TfR-GFP, vesicles were moved to the cell center in cells expressing FRB-3myc-KIF13B^832–1,826 but not FRB-3myc-KIF13B^442–831. The yellow lines outline the cell boundaries. Bar, 30 µm.