Table 1.
Constructs used in the induced dimerization assay
| Construct | Construct designa | Accession number |
| FRB-KIF1A tail | FRB-3myc-YID-KIF1A391–1,698 | NM_008440 |
| FRB-KIF1Bβ tail | FRB-3myc-YKGGSGG-KIF1Bβ387–1,770 | NM_207682 |
| FRB-KIF13A tail | FRB-3myc-YKYSDLELKLRILQSTVPRA-KIF13A361–1,749 | NM_010617 |
| FRB-KIF13B442–1,826 (full length) tail | FRB-3myc-YKGGGSGGGSGGG-KIF13B442–1,826 | NM_001081177 |
| FRB-KIF13B442–831 (N-terminal fragment) tail | FRB-3myc-YKGGGSGGGSGGG-KIF13B442–831 | NM_001081177 |
| FRB-KIF13B832–1,826 (C-terminal fragment) tail | FRB-3myc-YKGGSGGSGG-KIF13B832–1,826 | NM_001081177 |
| GFP-BicD2-FKBP | GFP-MID-FLAG-SH-BicD21–594-RTVSR-FKBP | AJ250106 |
| tdTM-BicD2-FKBP | tdTM-MID-FLAG-SH-BicD21–594-RTVSR-FKBP | AJ250106 |
| BicD2-FKBP | FLAG-SH-BicD21–594-RTVSR-FKBP | AJ250106 |
| KIF5C-tdTM-FKBP | KIF5C1–559-ELGAPRPT-tdTM-FKBP | NM_001107730 |
| FRB-Rab5 | FRB-3myc-YKRTGSG-Rab5a | NM_025887 |
| FRB-Rab7 | FRB-3myc-YKRTGSG-Rab7 | NM_009005 |
Accession numbers were obtained from GenBank.
a
Includes epitope tag or label (FLAG, myc, GFP, or tdTM), heterodimerization domain (FRB or FKBP), amino acid linker sequence (using single letter code), and trafficking protein (amino acids included).