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. 2015 Feb 2;208(3):367–379. doi: 10.1083/jcb.201407101

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© 2015 Wright et al.

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Figure 2. — Embryonic Merkel cell precursors express Atoh1, are unipotent, and give rise to the adult Atoh1⁺ population. (A) Experimental paradigm, outcomes, and interpretations. (B–C″) Whole-mount immunostaining for K8 (B and C), endogenous tdTomato signal (B′ and C′), and merged images (B″ and C″) in E16.5 (B–B″) and E18.5 (C–C″) Atoh1^CreER-T2/+;ROSA^tdTomato embryonic body skin. (D) K8⁺ and tdTomato⁺ cell numbers increase from E16.5 to E18.5 (n = 3–6 mice, t test). Error bars show SEM. (E–F″) Body skin (E–E″) and whisker follicle (F–F″) from a 24-wk-old (P168) Atoh1^CreER-T2/+;ROSA^tdTomato mouse that received high-dose tamoxifen at E15.5. (G) The vast majority of K8⁺ cells in touch domes and whisker follicles were tdTomato⁺ at P28 (n = 2) and P168 (n = 1) after tamoxifen administration at E15.5. Error bars show SEM. (H–H″) Single confocal z slice of touch dome whole-mount preparation from an E18.5 K14^CreER;ROSA^tdTomato mouse given tamoxifen at E16.5 and immunostained for K8 (H) shows that the two signals are not colocalized. ***, P < 0.001. Bars, 50 µm.