Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Feb;25(2):155–166. doi: 10.1101/gr.176925.114

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Borck et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Figure 4. — In vitro transcription defects caused by BRF1 mutations in yeast. (Top) A representative gel of the in vitro transcription reaction using a nuclear extract harboring a deficient Brf1 protein (p.Trp107Arg, W107R) that is impaired in Pol III–dependent transcription. Addition of WT Brf1 rescues activity whereas the different Brf1 variants and the combinations present in the affected children show a defect in transcription. Data are presented as mean ± SD. A quantification is shown in the middle panel. (*) P < 0.01; (**) P < 0.0001. The lower panel shows a twelve times excess of the Brf1 protein amount used for this assay separated on an SDS gel.