Results for phospho-ERK, phospho-JNK, phospho-p38, phospho-Akt and phospho-CREB are determined using specific phospho-antibodies. The results are determined at the indicated times with CX3CL1 and MCP-1, and after a 5 min incubation with the positive controls, PDGF-BB (PDGF, top panel) or forskolin (For, bottom panel). This experiment is representative of 4 experiments.