Figure 3.

Activated B cells induce tumor reactive T cell responses in tumor-bearing hosts. Mice bearing 4T1 tumor were treated with activated B cells, IL-2 or no treatment. Spleens of the mice were harvested and splenic T cells activated in vitro. These activated splenic T cells were tested for tumor reactivity by measuring IFNγ release when mixed with irradiated tumor cells. Mice that received activated 4T1 TDLN B cells were found to have splenic T cells reactive to 4T1 tumor cells, and not to irrelevant tumor cells indicating the ability of activated B cells to present antigen to the secondary host. (Li et al.)⁴⁰