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Activated TDLN B cells produce antibody that can mediate complement dependent cytotoxicity (CDC). Supernatants (Sup) from activation cultures of D5 and MCA 205 TDLN B cells were tested for their ability to mediate CDC in vitro. Pan-02 was used as an irrelevant tumor cell control. As can be seen, the supernatants contained antibodies that were able to specifically lyse the relevant tumor targets. (Li et al.)39
