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TDLN B cell-produced IgG2b binds specifically to tumor cells. D5, MCA 205, and Pan-02 tumor cells were incubated with the culture supernatant (Sup) of MCA 205 TDLN B cells. Bound IgG2b onto tumor cells was then detected by a secondary antibody, FITC-anti-mouse IgG2b. FITC-coupled isotype-matched control was used to measure background staining. (Li et al.)39
