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Antibodies produced by activated D5 TDLN B cells can bind to unique epitopes from the lysate of D5 tumor cells and not to other tumor lysates. Supernatants from activated D5 TDLN T cells and B cells were admixed with the lysates of D5, MCA 205 and Pan-02 tumor cells. The immunoprecipitates were then run on an SDS-PAGE gel. Unique bands were identified in the lane were antibodies produced by activated D5 TDLN B cells were admixed with D5 tumor lysate.(Namm et al. unpublished data).
