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. 2015 Feb 3;10(2):e0112305. doi: 10.1371/journal.pone.0112305

Figure 1. Methylation status of CpG islands in the promoter regions of Cosmc gene.

Figure 1

DNA methylation levels of CpG islands in the promoter regions of Cosmc gene were detected by bisulfite-modification sequencing PCR, and high-throughput quantitative DNA methylation analysis. Samples are B lymphocytes from peripheral blood of the 3 groups (IgAN patients, n = 26; other renal disease patients, n = 11; healthy children participants, n = 13). Error bars represent s.e.m. and * denotes a p-value ≤0.05. * *≤ 0.01. * * *for p-value ≤ 0.001. (a) DNA methylation in 3 groups after 48 hours culturing. (b) DNA methylation in 3 groups after 48 hours culturing with IL-4 (10ng/ml). (c) DNA methylation in 3 groups after 48 hours culturing with AZA (1umol/l). (d) DNA methylation in 3 groups treated or not (beige) with IL-4 (green) or AZA (blue). (e, f) Compared to plain medium, IL-4 or AZA caused the alteration of DNA methylation levels in the other two groups.